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KMID : 0357119940160030227
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Korean Journal of Immunology
1994 Volume.16 No. 3 p.227 ~ p.236
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The Role of Interferon-γ and Prostaglandin E2 in the Production of Tumor Necrosis Factor-α Mouse Macrophages Stimulated with Lipopolysaccharide
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Kim Joong-Gon
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Abstract
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The roles of interferon-γand prostaglandin E2 in the regulation of tumor necrosis factor-αproduction by mouse peritoneal macrophages stimulated with lipopolysaccharide were investigated. The production of TNF-α by mouse macrophages stimulated with LPS reached the peak level 3-6 hours after the in- itiation of culture and then rapidly decreased with time. IFN-γ increased the amount of and prolonged the duration of the production of TNF-α by macrophages in response to LPS. However, this enhancing effects of INF-α were not observed if IFN-γ were given to the macrophages 2 hours after the addition of LPS, suggesting that macropahges activated with LPS became resistant to enhancing effects of IFN-γ. PGE2 suppressed the production of TNF-α by macrophages stimulated with LPS, but IFN-γ reduced the suppressive effects of PGE2 on the TNF-α production by having macrophages resistant to the suppressive effect of PGE. Indomethacin, inhibitor of cyclooxygenase, enhanced the production of TNF-α in response to LPS or LPS and IFN-γ, suggesting that the endogenous PGE2 produced by macrophage stimulated with LPS might play a role in the suppression of TNF-α production in response to LPS. Collectively, the balance between the up-regulatory effect of IFN-γproduced by T lymphocytes and down-regulatory effect of PGE2 produced by macrophage might play an important role in regulating the production of TNF-α by mouse macrophages in response to LPS
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KEYWORD
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Mouse macrophage LPS TNF-α IFN-γ PGE2 indomethacin
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